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Acclimatization of Tissue Cultured Sengon: From In Vitro To Field
Asri Insiana Putri

Last modified: 2019-05-24

Abstract


Vegetative propagation is necessary to maintain the genetic material of sengon (F. moluccana) for the xylem and phloem differentiations or disease tolerance. The ultimate success of micro propagation on a commercial scale depends on the ability to acclimatization plants out of in vitro culture on a large scale to field with high survival rates. In order to increase growth and reduce mortality in plantlets at the acclimatization stage, efforts are focused on the control of both abiotic and biotic seedlings environment of micro cutting from in vitro condition.Compost and soil as seedlings medium used for biotic treatments. Temperature and moisture condition of seedlings environment used as abiotic treatments. Tissue cultured (TC) sengon compare with non-tissue cultured or local seedlings (NTC) used for field acclimatization observation. The aims of this study were to observe acclimatization of tissue cultured sengon from in vitro to field: 1) acclimatization of explants from micro cutting in vitro to greenhouse and nursery, 2) acclimatization seedlings ex vitro to the field. TC has belter growth after 2 years old acclimatization in the field

 


Keywords


Keywords: sengon, acclimatization, rooting ability, field mortality

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