Research, Development and Innovation Agency Conference Portal, International Conference on Managing Forest Genetic Resources in Changing Environment and Landscapes

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Genetic structure of Giam populations in Sumatera based on RAPD Markers
AYPBC Widyatmoko

Last modified: 2019-05-23


Giam (Cotylelobium melanoxylon) that naturally distributed in Sumatera was listed in IUCN Red List as Threatened Species because individual and population number were decreased. Therefore, the conservation of giam is needed and the information of the genetic structure of this species is required to support the conservation strategy. A genetic study using RAPD markers was conducted to obtain this information. Thirty seven samples from two populations, i.e. Hutan Adat Guguk (Jambi) and PT. Kojo (Riau) were used in this study. Eight RAPD primers were selected and produced 36 polymorphic loci. Genetic diversity of Hutan Adat Guguk population and PT. Kojo population were 0.3958 and 0.3564, respectively, while genetic distance between two populations was 0.2829. A specific locus was found in PT. Kojo population (A1-1000), and several specific alleles were found in each population. Genetic diversity of giam was considered as high despite the decline of individual and population number. Genetic distance between two populations was also high and correlated with their geographic distance. Both populations have specific locus and allele that differentiate the populations. This information should be taken into account for sample collection strategy and establishment of ex-situ conservation plots.

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