Research, Development and Innovation Agency Conference Portal, International Conference on Managing Forest Genetic Resources in Changing Environment and Landscapes

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Nondestructive and Destructive Method for COI gene Isolation from Coleoptera
anindya safita ningtias, Istiana Prihatini

Last modified: 2019-05-24

Abstract


It is known that the distribution of Ceratocytis is vectored by insects. Identification of insect species through a molecular approach is one of the first steps in managing control of Ceratocystis. DNA extraction, which is an important step in molecular identification, always involves the process of destruction, whether part or all parts of the body. This process causes the loss of body parts that are important for the key to determination. So that, research related to non-destructive methods began. The aimof this researchwas to find the most effective method for isolating the COI gene and amplifying the gene throughpolymerase chain reaction (PCR). Coleoptera samplescollected from Acacia spp.plantsin Pelalawan Riau. Extraction and PCR were carried out at the Molecular Genetic Laboratory of the Centre for Forest Biotechnology and TreeImprovement Research in Yogyakarta. Thosesamples were extracted using the CTAB method with 4 incubation treatments, that weresoaking,crushing, soaking-crushing and freezing-crushing.Primer pair of LCO1490-HCO2196 and LCO1490-HCO2198 were used to amplify the COI gene. The COI gene is not amplified in the soaking(non-destructive) treatment in both primers.


Keywords


Ceratocystis; Coleoptera; COI; DNA extraction; non-destructive

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